Tripeptide derivatives
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United States of America Patent
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Jan 31, 1984
Grant Date -
N/A
app pub date -
Mar 25, 1981
filing date -
Mar 25, 1981
priority date (Note) -
Expired
status (Latency Note)
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Abstract
Tripeptide derivatives which are useful as substrates for assaying proteolytic enzymes. The said derivatives are compounds of formula H--D--X--Y--Z--R wherein X represents a cyclohexylglycyl, cyclohexylalanyl, p-hydroxycyclohexylalanyl, phenylglycyl, phenylalanyl, tyrosyl, leucyl, isoleucyl, norleucyl, valyl, norvalyl, .alpha.-aminobutyryl, alanyl, prolyl or pipecolinoyl group, Y represents a cyclohexylglycyl, cyclohexylalanyl, p-hydroxycyclohexylalanyl, phenylglycyl, phenylalanyl or tyrosyl group and, if the meaning of X is limited to cyclohexylglycyl, cyclohexylalanyl, p-hydroxycyclohexylalanyl, phenylglycyl, phenylalanyl or tyrosyl, additionally a leucyl, isoleucyl, norleucyl, valyl, norvalyl, .alpha.-aminobutyryl, alanyl, prolyl or pipecolinoyl group, Z represents an arginyl or lysyl group and R represents a chromogenic group which can be split off by enzymatic hydrolysis and which is capable of forming a colored or fluorescent compound. The tripeptide derivatives are preferably used in the form of their salts with acids.
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