Enzyme production and purification
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United States of America Patent
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Aug 16, 1983
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N/A
app pub date -
Jun 26, 1981
filing date -
Jul 5, 1980
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Expired
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Abstract
There is described a process for the production of a polysaccharide-producing enzyme, which comprises fermenting an appropriate enzyme producing organism in aqueous sucrose, characterized in that the sucrose concentration is maintained at between 1 and 10 g/l throughout all but the last part of the fermentation and that the dissolved oxygen content of the fermentation is controlled. There is also described a method for the purification of a polysaccharide producing enzyme, which comprises absorbing a mixture containing the enzyme on an anionic resin and removing the enzyme from the resin with a sugar containing eluant, and a novel highly purified form of polysaccharide producing enzyme.
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