Process for producing the enzyme cholesterol esterase and for hydrolyzing cholesterol esters of fatty acids by using the enzyme itself
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United States of America Patent
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Jun 7, 1983
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N/A
app pub date -
Jul 13, 1981
filing date -
Jul 24, 1980
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Expired
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Abstract
The enzyme cholesterol esterase is produced by cultivating a strain of the Achromobacter delicatulus type, isolated from vegetable debris and identified by the symbol NRRL B-12115. Under the described culture conditions, the enzyme is exocellular and therefore does not require extraction from the cells. In addition, the micro-organism has a duplication time less than that of moulds and Actinomycetes. Cholesterol esters of fatty acids can be hydrolysed by adding them to the culture medium before inoculating the strain.
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