Enzyme immunoassay for determining antigen specific antibodies and test kit for carrying out this assay
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United States of America Patent
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Aug 31, 1982
Grant Date -
N/A
app pub date -
Jul 2, 1980
filing date -
Jul 28, 1979
priority date (Note) -
Expired
status (Latency Note)
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Abstract
A highly sensitive enzyme immunoassay procedure is disclosed for determining IgM-, IgA-, IgG- and IgE- antibodies which are specific to antigens by coating a solid support with an antibody which selectively binds the antibody to be determined in a serum specimen and reacting the selectively bound antibody with an enzyme labeled microbe or antigen and then determining the enzyme activity of the reaction product as a measure of the antibody to be detected. As the antigen can be effectively labeled a relatively impure antigen can be labeled and used in high dilutions resulting in a very sensitive assay; purification by column chromatography is unnecessary. A kit for conducting the assay is also disclosed.
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