Production of immobilized glucose isomerase
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United States of America Patent
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Aug 10, 1982
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N/A
app pub date -
Dec 19, 1980
filing date -
Feb 5, 1980
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Expired
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Abstract
An immobilized glucose isomerase is prepared by treating an aqueous suspension of cells of a glucose isomerase producing microorganism with a non-ionic surfactant that solubilizes glucose isomerase contained by the cells without solubilizing polysaccharides in the cells, separating the cells from the aqueous suspension, and adsorbing glucose isomerase from the resultant solution onto an ion exchange resin. By this process, contaminating polysaccharides are eliminated that inhibit adsorption of glucose isomerase on the ion exchange resin.
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