Chemiluminescent enzyme immunoassay
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United States of America Patent
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Nov 24, 1981
Grant Date -
N/A
app pub date -
Mar 7, 1978
filing date -
Mar 16, 1977
priority date (Note) -
Expired
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Abstract
A heterogeneous enzymatic immunoassay, in which chemiluminescence is employed as a detection means, is disclosed. The chemiluminescence is produced by an enzymatic catalyzed redox reaction between hydrogen peroxide and a phenolic compound such as pyrogallol, resorcinol, phloroglucinol and hydroquinone. The assay is disclosed as having sensitivity equal to that of radioimmunoassays and reverse hemaglutination tests without some of the drawbacks of these methods, e.g., radioactivity hazards.
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