Assaying of digoxin by means of bioluminescence and inhibition of NA.sup.+-.sup.+ -ATPase

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United States of America Patent

PATENT NO 4264727
SERIAL NO

06060006

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Abstract

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A method is described for quantitative measurement of digoxin and other digitaloids in serum. The method is based on the inhibition of Na.sup.+, K.sup.+ --specific ATPase enzyme by digitaloids as a result of receptor binding principle and the measurement of this inhibition with the firefly luceferin--luciferase bioluminescence assay of APT. Method is specific and has similar sensitivity to that of the commonly used radioimmunoassay, but simpler and can be performed with low-cost instrumentation.

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Patent Owner(s)

  • MINNESOTA MINING AND MANUFACTURING COMPANY;LUMAC INTERNATIONAL N.V.,, A CORP. OF THE NETHERLANDS ANTILLES

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Inventor(s)

Inventor Name Address # of filed Patents Total Citations
Kolehmainen, Seppo Ganzenstraat 11, 3540 Zolder, BE 5 114
Tarrkkanen, Veikko Breulsweg, Wylre 1, NL 1 8

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