Substrates for angiotensin converting enzyme
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United States of America Patent
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-
Feb 17, 1981
Issued Date -
N/A
app pub date -
Mar 14, 1978
filing date -
Mar 14, 1978
priority date (Note) -
Expired
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Abstract
A method is disclosed for quantitative measurement of angiotensin converting enzyme activity in biological material. The method exploits certain acylated tripeptide substrates. The enzyme-catalyzed hydrolysis of the substrates results in the formation of a dipeptide reaction product and a remnant product. The substrate is radioactively labeled exclusively in that portion destined to become the remnant product. Preferably, the substrate prior to hydrolysis is essentially insoluble in an aprotic organic solvent, but the remnant hydrolysis product is essentially quantitatively extractible by the organic solvent. At the termination of the enzyme-catalyzed hydrolysis, the labeled remnant product is separated from the reaction mixture, and the radioactivity is counted in a suitable apparatus.
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