Composition and method for the determination of cholesterol
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United States of America Patent
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Jan 1, 1980
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N/A
app pub date -
Oct 13, 1977
filing date -
Oct 29, 1976
priority date (Note) -
Expired
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Abstract
In a process for the determination of total or bound cholesterol by liberating any bound cholesterol using cholesterol esterase and simultaneously or subsequently determining the liberated cholesterol and any initially free cholesterol with a cholesterol reacting enzyme, the improvement comprising using as the cholesterol reacting enzyme an NAD or NADP-dependent cholesterol dehydrogenase obtained from an anaerobic microorganism, e.g., Eubacterium sp. ATCC 21408, or from mammalian tissue, e.g., liver.
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