CONSTANT TEMPERATURE NUCLEIC ACID AMPLIFICATION RAA PRIMER PROBE COMBINATION FOR DETECTING CANDIDA AURIS AND USE THEREOF
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United States of America
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Apr 10, 2025
app pub date -
Oct 8, 2023
filing date -
Oct 8, 2023
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Abstract
The present invention discloses a constant temperature nucleic acid amplification RAA primer probe combination for detecting Candida auris and use thereof are provided. The primer probe combination has high amplification efficiency, strong specificity, and no obvious false positive, and can effectively distinguish Candida auris from three closely related bacteria frequently misdiagnosed in a clinical microorganism identification system. Based on an RAA-lateral flow chromatography technology, the present invention establishes a method for rapidly detecting Candida auris can be used for detecting an actual sample after passing the specificity and sensitivity evaluation, and provides a rapid, sensitive, reliable, and effective new method for on-site rapid detection of Candida auris. The method does not need complex instruments, and is particularly suitable for rapid screening and detection of Candida auris in basic laboratories and quarantine sites.
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