PROGRAMMABLE NUCLEASE-BASED ASSAY IMPROVEMENTS
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United States of America
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Apr 10, 2025
app pub date -
Jun 12, 2024
filing date -
Jun 12, 2024
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Abstract
The present disclosure describes various improvements for programmable nuclease-based detection assays. Also provided are compositions, methods, kits, systems, and devices for practicing the same. Such improvements include improved reporter designs to facilitate the cleavage of a reporter immobilized on a substrate by an activated programmable nuclease. Improved reporter designs may comprise various lengths and structures of the reporter. The substrate can also comprise immobilized guide nucleic acids and/or programmable nucleases.
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