Importance

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Abstract

A single-stranded oligonucleotide labeled with a fluorescent substance and a quenching substance. In the single-stranded oligonucleotide, a hairpin structure is formed by self-hybridization on the 5′ side, and when a single-stranded oligonucleotide having a complementary base sequence on the 3′ side hybridizes, a triplex structure is formed on the 5′ end portion, and cleaved by a flap endonuclease that recognizes the triplex structure, whereby the fluorescent substance is released from the quenching substance, the fluorescent substance emitting fluorescence when irradiated with excitation light, and the single-stranded nucleotide is a single-stranded oligonucleotide including a non-complementary base pair within the hairpin structure, a single-stranded oligonucleotide in which a nucleotide residue labeled with the fluorescent substance and a nucleotide residue labeled with the quenching substance are separated from each other by 4 or more residues, or a single-stranded oligonucleotide in which the fluorescent substance is bound to a portion other than the base.

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First Claim