SCREENING METHOD FOR ANTI-PSEUDOMONAL AGENTS AND COMPOSITIONS AND METHODS FOR TREATING PSEUDOMONAS INFECTIONS
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United States of America
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Apr 10, 2025
app pub date -
Oct 13, 2022
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Oct 13, 2022
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Abstract
Methods of screening for anti-pseudomonal compositions are disclosed that utilize a genetically engineered P. aeruginosa strain that is recA deficient and comprises a transcriptional reporter joined to at least a portion of a promoter for at least one gene of a pyocin gene cluster. The methods identify agents that stimulate pyocin expression in a RecA-independent manner. Also disclosed are agents identified by said methods, along with methods of producing and using same.
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