METHODS FOR QUANTITATING VIRAL CAPSIDS
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United States of America
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Apr 3, 2025
app pub date -
Feb 28, 2024
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Feb 28, 2024
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Abstract
Methods for quantitating viral capsids and/or polynucleotides contained therein using a charged biopolymer-loaded biosensor are provided. The signals indicative of binding of molecules (such as polynucleotides or viral capsids) to the biosensor can be measured by bio-layer interferometry (BLI). The amount of viral capsids can be calculated based on signals indicative of binding of the viral capsids to the biosensor. The empty/full viral capsid ratio in a viral sample can be calculated based on the amount of the viral polynucleotides and/or the binding kinetics of the biosensor to the viral capsids or the polynucleotides.
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