HIGH SENSITIVITY BIOLAYER INTERFEROMETRY WITH ENZYME AMPLIFICATION
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United States of America
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Apr 3, 2025
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Oct 16, 2024
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Oct 16, 2024
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Abstract
The present invention provides a high sensitivity immunoassay method for determining the concentration of an analyte in a sample based on interferometry. The present invention provides a high molecular weight conjugate comprising multiple streptavidins and horse radish peroxidases (HRP) and uses this conjugate to increase the sensitivity of a biochemical assay. The high molecular weight conjugate comprises at least 2 HRPs, preferably 4 HRPs and binds a precipitating HRP substrate to a solid phase to increase the signal of a biochemical assay.
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