INTEGRATING SPHERE-BASED FLUORESCENCE DETECTION SYSTEM FOR REAL-TIME QUANTITATIVE PCR DEVICE
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United States of America
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Apr 3, 2025
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Sep 30, 2023
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Sep 30, 2023
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Abstract
The present invention is an integrating sphere-based fluorescence detection system for real-time quantitative PCR device comprising various components, including PCR sample reaction plate, illumination sources configured to produce excitation light, detection source configured to detect fluorescence emission light produced, in response to the excitation light from the sample reaction plate, an integrating sphere configured to direct the excitation light to the sample plate uniformly and to direct the fluorescence emission light from the sample holder along a response path to the imaging module. The system may enhance the quality of excitation light hitting samples in the sample holder.
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