METHOD FOR DETECTING MULTIPLE TARGET NUCLEIC ACIDS
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United States of America Patent
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Issued Date -
Mar 27, 2025
app pub date -
Dec 27, 2022
filing date -
Dec 27, 2022
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Abstract
The present invention relates to the field of molecular biology, in particular to a method for detecting multiple target nucleic acids in a single sample container. The present invention particularly relates to a method for multiplex detection based on digital PCR. The method comprises: mixing a primer-probe composition, a sample to be detected and an amplification reagent to obtain a reaction system; placing the reaction system in a condition that allows a nucleic acid polymerase to perform hybridization and extension reaction, so as to obtain a reaction product; and performing signal acquisition on the reaction product. The detection method of the present invention can realize detection of multiple target nucleic acids, is easy to operate, and can save time.
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