TERMINAL TRANSFERASE VARIANTS FOR CONTROLLABLY SYNTHESIZING SINGLE-STRANDED DNA AND USE THEREOF
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Mar 27, 2025
app pub date -
Jan 13, 2023
filing date -
Jan 13, 2023
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Abstract
A plurality of terminal deoxynucleotidyl transferases (TdTs) and variants thereof are used for de novo synthesis of polynucleotides having controlled sequences and efficiently controllable synthesis of nucleic acid molecules without depending on a template. It is found that some amino acid residues in the TdT catalytic domain can be specifically modified to improve the ability of such modified TdTs to synthesize polynucleotides.
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