METHOD FOR PREPARING BROWN PLANTHOPPER RESISTANT PLANTS USING RICE BROWN PLANTHOPPER RESISTANCE GENE BPH33.2
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United States of America Patent
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Mar 27, 2025
app pub date -
Jan 17, 2024
filing date -
Jan 17, 2024
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Abstract
The present disclosure locates a new brown planthopper resistance gene BPH33.2 on the short arm of rice chromosome 4 by extreme mixed pool analysis (BSA-seq), and genetically transforming the gene BPH33.2 to make the susceptible rice to be the brown planthopper resistant phenotype rice; at the same time, the knockout of the gene BPH33.2 was used to cause the loss of the brown planthopper resistant phenotype in insect resistant rice, thus confirming the function of BPH33.2. The present disclosure also provides molecular markers closely linked with the rice brown planthopper resistance gene BPH33.2. Through experimental detection of molecular markers linked or co-segregated with these resistance loci, the brown planthopper resistance of rice plants can be accurately predicted at the seedling stage, accelerating the progress of the selection of brown planthopper-resistant rice varieties.
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