METHOD FOR PRODUCING A BIOPRODUCT
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United States of America Patent
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Issued Date -
Mar 27, 2025
app pub date -
Jul 21, 2022
filing date -
Jul 21, 2022
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Abstract
Various embodiments provide a method for producing a bioproduct using a bioprocess installation. The bioprocess installation comprises an process control and a bioprocess unit. The bioprocess unit comprises a receptacle, a clarification unit with a centrifuge and a chromatography unit with a chromatograph. Cell broth obtained from the receptacle is lead through the clarification unit and the chromatography unit in a liquid stream. The clarification unit with its centrifuge is being operated in a centrifugation cycle comprising centrifugation steps. The chromatography unit with its chromatograph is being operated in a chromatography cycle comprising chromatography steps. The particle depletion can be less than 10% in particle concentration and that the execution of the steps assigned to the centrifugation cycle and the execution of the steps assigned to the chromatography cycle are at least partly being synchronized with each other by the process control in synchronization routines based on assigned synchronization strategies.
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