SINGLE-LOCI AND MULTI-LOCI TARGETED SINGLE POINT AMPLICON FRAGMENT SEQUENCING

Number of patents in Portfolio can not be more than 2000

United States of America

APP PUB NO 20250095782A1
SERIAL NO

18780156

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Abstract

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The systems and methods described herein are directed to amplifying microbial cell free DNA (mcfDNA). In an aspect, described herein is a method of amplifying microbial cell free DNA (mcfDNA), comprising using one or more degenerate primers with complementarity to one or more conserved regions and a second primer comprising complementarity to a repaired version of an adaptor ligated to ends of the mcfDNA, wherein the one or more degenerate primers are oriented to prime polymerase extension of the hypervariable region to generate amplified mcfDNA fragments.

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Patent Owner(s)

Patent OwnerAddress
GUSTO GLOBAL LLC2025 CASSAMIA PL CHARLOTTE NC 28211

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Inventor(s)

Inventor Name Address # of filed Patents Total Citations
Ouellette, Lisa Huntersville, US 1 0
Taghavi, Safiyh Chapel Hill, US 27 112
Van, Der Lelie Daniel Chapel Hill, US 39 324

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