Methods for Detecting and Discriminating among Microbes in Microbial Pathogen Mixtures
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United States of America
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Mar 20, 2025
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Sep 20, 2024
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Sep 20, 2024
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Provided herein are methods for detecting and discriminating bacteria and fungal species by nucleic acid amplification, then hybridization analysis on a microarray. Primer pairs are used to simultaneously amplify multiple ribosomal Hypervariable regions from the rDNA or rRNA in bacteria and/or fungi as a multiplex amplification reaction, wherein the resulting hypervariable region amplicon mix is analyzed by hybridization to nucleic acid probes that are complementary to unique sequence determinants within those amplified hypervariable regions. The combination of multiplex amplification and microarray hybridization is used for detection and differentiation of specific species within a microbial set relevant to human or animal health or environmental or agricultural analysis, based on simultaneous analysis of the sequence in two or more hypervariable regions, concurrently.
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