A METHOD OF FORMING A COMPOSITION COMPRISING A PROBIOTIC MICRO-ENCAPSULATED IN A DENATURED PLANT PROTEIN MATRIX
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United States of America
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Mar 20, 2025
app pub date -
Jan 27, 2023
filing date -
Jan 27, 2023
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Abstract
A method of forming a composition comprising a probiotic encapsulated in a denatured plant protein matrix, comprises the steps of preparing a protein suspension comprising denatured plant protein, preparing a suspension of hydrated probiotic, combining the protein suspension and the suspension of hydrated probiotic to encapsulate the probiotic in a denatured pea protein matrix, and polymerising the denatured plant protein matrix with a calcium salt. The combining step may comprise extruding the protein suspension and the suspension of hydrated probiotic to form microdroplets, in which the polymerisation step comprises curing the extruded microdroplets in a curing bath comprising a calcium citrate buffer having a pH of 5 to 6.5 and a molarity of 0.05 to 0.15 M. The combining step may also comprise mixing the protein suspension and probiotic suspension to form a mixture, adding a calcium salt buffer to the mixture to gel the mixture, and drying the gelled mixture by freeze-drying or vacuum drying.
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