METHOD FOR OPTIMIZING MRNA SEQUENCE USING PEPTIDE BARCODE
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United States of America
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Mar 6, 2025
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Aug 1, 2024
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Aug 1, 2024
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Abstract
There is provided a method for optimizing a nucleic acid sequence, including the steps of: preparing a nucleic acid sequence that includes a candidate sequence containing a sequence of an untranslated region and a sequence encoding a target protein, and a sequence encoding a peptide barcode directly or indirectly linked to the target protein; expressing a protein from the nucleic acid sequence; separating the peptide barcode from the protein; analyzing the separated peptide barcode; and acquiring a relationship between expression of the target protein and the candidate sequence based on a result of the analysis, and selecting an optimal candidate sequence.
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