A BIOASSAY MODULE, A KIT FOR THE DETECTION OF BACILLUS CEREUS AND METHODS THEREOF
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United States of America
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Mar 6, 2025
app pub date -
Jan 3, 2023
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Jan 3, 2023
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Abstract
The present invention teaches a bioassay module for detection of pathogenic microorganisms comprising: food specimen deposit element; indicator layer comprising dye; enzymatically digestible substrate layer, forming a barrier between the food specimen deposit element and indicator layer; isolation layer, separating the enzymatically digestible substrate layer from layers underlying the enzymatically digestible substrate layer; separation layer; absorption layer to absorb and display the indicator; wherein the layers are disposed from bottom to top the absorption layer, the separation layer, the indicator layer, the isolation layer, the enzymatically digestible substrate layer, the food specimen deposit element, and enzymatically digestible substrate layer is liquefiable and perforable by enzymes secreted by the pathogenic microorganism in contaminated food disposed in the food specimen deposit element, the digested substrate layer is flowable to the indicator layer via the isolating layer, the liquefied dye flowable to the absorption layer and visible at least from the top layer.
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