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Abstract

This invention relates to the field of genetic molecular breeding, specifically to a pig 10K liquid-phase chip based on multiple single nucleotide-polymorphism and its application. The present invention, while adhering to the basic principles of liquid phase chip design, adds new markers to existing chips and optimizes probes using multiple single nucleotide-polymorphism technology. This results in the generation of more SNP markers with high genotyping quality and moderate linkage disequilibrium with target SNP loci within the probe region, thereby increasing the effective marker count of the chip. The mSNP liquid phase chip of the present invention increases the number of detectable SNPs to 1.5-2 times the number of target SNP markers, addressing the issue of existing chips that only contain target SNPs and cannot provide mSNP markers, without increasing the cost. By using genotype imputation technology, genotype data from the chip of the present invention is imputed into mainstream 50K chips for genomic selection, achieving molecular breeding accuracy comparable to or similar to that of the 50K chips, thereby reducing the cost of pig molecular breeding.

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First Claim