METHOD OF BACTERIAL IDENTIFICATION AND TESTING OF BACTERIUM RESISTANT TO ONE OR MORE ANTIBIOTICS
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United States of America
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Feb 20, 2025
app pub date -
Dec 29, 2022
filing date -
Dec 29, 2022
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Abstract
Disclosed herein is a method of detecting an antimicrobial susceptibility of a bacterium to one or more antibiotics, the method requiring the steps of: (a) providing a bacterial mixture that includes a bacterial population including a bacterial species suspected to be resistant to one or more antibiotics, an antibiotic, a metabolic precursor suitable for incorporation into a bacterium, which metabolic precursor is labelled or is capable of being labelled with a detectable moiety, and a nutrient suspension; and (b) aging the bacterial mixture for a period of time, then collecting the bacterial population and resuspending it to provide an antibiotic-challenged bacterial mixture, where a bacterium that is resistant to one or more antibiotics incorporates the metabolic precursor suitable for incorporation into the bacterium.
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