REGULATORY NUCLEIC ACID MOLECULES FOR MODIFYING GENE EXPRESSION IN CEREAL PLANTS
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United States of America
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Feb 20, 2025
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Dec 22, 2022
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Dec 22, 2022
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The present invention relates to the field of plant molecular biology and provides materials and methods for modulating expression of a gene of interest in plants. In particular, the invention provides modified plant promoters or modified coding sequences having increased expression, for example, in developing spikes as well as methods for producing promoters or coding sequences having increased expression. The modified promoters comprise i) at least one binding site for an EIL3 transcription factor and/or at least one binding site for a PHD transcription factor and/or ii) one or more enhancer elements. Moreover, the present invention concerns a nucleic acid molecule encoding a functional restorer polypeptide for wheat G-type cytoplasmic male sterility comprising in the coding sequence a mutated microRNA (“miRNA”) binding site. In some embodiments, said nucleic acid molecule is operably linked to the modified promoter of the present invention.
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