SINGLE PARTICLE ANALYSIS METHOD FOR VIRAL NUCLEIC ACID VECTOR
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United States of America
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Feb 13, 2025
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Oct 9, 2024
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Oct 9, 2024
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Abstract
The present disclosure relates to a method for analyzing one or more parameters of a viral nucleic acid vector, and belongs to the field of biological technology. The method may optionally comprise preparing a particle size standard curve and/or a concentration standard solution for the viral nucleic acid vector to measure the particle size and the concentration thereof. The method comprises preparing a specific target recognition reagent conjugated to a fluorescence labeling reagent for the parameters of the viral nucleic acid vector, detecting a sample by a flow particle analyzer, and the like. The method enables a deeper analysis of the “viral titer” obtained by traditional approaches, yielding more accurate detection results, which is of significant importance for downstream release decisions, process control, and the evaluation of clinical safety.
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