IMPROVED SCREENING METHOD FOR GENOME EDITED EVENTS
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United States of America
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Feb 13, 2025
app pub date -
Dec 20, 2022
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Dec 20, 2022
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Abstract
The present invention is in the field of plant molecular biology and is directed to a method for improved screening for known edits within the genome of a cell. The method of the invention comprises dividing a population of plant cells comprising a desired nucleic acid sequence, into subgroups, quantifying the concentration of the desired nucleic acid sequence for each sub-group, culturing cells from the a sub-group(s), dividing the cells into subgroups, quantifying the concentration of the desired nucleic acid sequence in each subgroup, and regenerating intact individual plants from the cells of a selected subgroup(s) with the desired nucleic acid sequence.
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