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Abstract

Disclosed is a method for gene expression by transient transformation of a wild rice seed using Agrobacterium. The method includes the following steps: subjecting a wild rice seed to induction culture and subculture in sequence, and selecting a resulting callus with a dense structure to allow pre-culture to obtain a pre-cultured callus; transforming a 1305Ubi-Ubi-GFP-H plasmid carrying a green fluorescent protein (GFP) gene into an Agrobacterium strain EHA105 or LBA4404 to obtain a positive Agrobacterium strain; subjecting the positive Agrobacterium strain to culture to obtain a bacterial suspension with an optical density (OD) value of 0.02 to 0.5 at a wavelength of 600 nm to obtain an infection bacterial solution; and infecting the pre-cultured callus with the infection bacterial solution to allow co-culture and recovery culture in sequence; observing an infection status of a recovered callus under a stereo fluorescence microscope, and calculating a transient expression rate.

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