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Abstract

This description relates to a substance causing a mutation at position 727 in an amino acid residue of the pAPN protein in any of the following: 1) constructing a cell line with site-directed modification of the pAPN gene for non-disease diagnostic and therapeutic purposes; 2) preparing a cell with resistance to the porcine transmissible gastroenteritis virus; and so on. A system for site-directed modification of the pAPN gene may effectively cleave two target sites of the pAPN gene, thereby achieving the precise mutation at position 727 in an amino acid of pAPN. Based on the precise modification of the pAPN gene while capable of avoiding disruption or alteration of the normal expression of other amino acids in the pAPN, the physiological activity function of pAPN protein on the basis of resisting TGEV infection is retained.

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First Claim