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Abstract

The invention provides an oligosaccharide-fluorescent marker, and a preparation method and use thereof. The method includes reacting an oligosaccharide with a fluorescent agent in a solvent in the presence of a reducing agent to obtain the oligosaccharide-fluorescent marker. According to the oligosaccharide-fluorescent marker of the invention, an amino group in the fluorescent agent is subjected to a condensation reaction with a hemiacetal group at a reducing end of the oligosaccharide, to form an imine or Schiff base, and then the imine or Schiff base is reduced into a relatively stable secondary amine by using the reducing agent. In this way, the end of the oligosaccharide is fluorescently marked. The fluorescent group marked at the end has small influence on the biological activity of the oligosaccharide, and the marker has higher fluorescence intensity and is stable under physiological conditions.

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First Claim