BACILLUS EXPRESSION SYSTEM
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United States of America
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Issued Date -
Feb 6, 2025
app pub date -
Dec 6, 2019
filing date -
Dec 6, 2019
priority date (Note) -
Published
status (Latency Note)
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Abstract
An inducible promoter expression system based on the T7 RNA Polymerase (T7 RNAP), lactose repressor (Lad), and a chimeric T7lac promoter (PT7lac), which can be integrated as a single copy into the B. subtilis genome. In the absence of IPTG. Lad strongly represses T7RNAP and PT7lac, and expression of an exemplary ORF—here superfolder green fluorescent protein (sfGFP) reporter protein—is undetectable by flow cytometry. Addition of IPTG de-represses PT7lac, and simultaneously induces expression of T7RNAP, resulting in very high sfGFP levels.
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