COMPOSITIONS AND METHODS FOR PURIFICATION AND DETECTION OF HDL AND APOAI
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United States of America Patent
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Jan 23, 2025
app pub date -
Sep 9, 2024
filing date -
Sep 9, 2024
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Abstract
The present invention provides methods, kits, and compositions for purifying HDL molecules from a sample (e.g., blood sample) using HDL tagging molecules comprising an HDL lipophilic core binding peptide (e.g., portion of ApoA1) and an affinity tag. The present invention also provides methods, kits, and compositions for detecting non-fragmented ApoA1 with mass spectrometry. The present invention further provides methods, kits, and compositions for tagging HDL molecules in a sample with detectably labeled ApoA1 molecules such that the ratio of detectably labeled ApoA1 molecules to native ApoA1 proteins may be determined.
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