MULTIPLEX CELLULAR ASSAYS FOR SCREENING AND QUALITY ASSESSMENT OF ENGINEERED CELLS
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United States of America Patent
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Issued Date -
Jan 23, 2025
app pub date -
Sep 20, 2024
filing date -
Sep 20, 2024
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The invention is directed to methods and systems for carrying out one or more highly multiplexed cellular assays. In some embodiments, one or more channels of a fluidic device are provided with photopolymerizable polymer precursors and cells randomly disposed on a surface, after which positions of cells are measured by a detector, hydrogel chambers are synthesized by photopolymerization to enclose individual cells, and channels are loaded with assay reagents. Assay signals indicative of desired cellular characteristics can be generated for each of the enclosed cells.
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