Compositions, Systems, and Methods for Performing a Mannich Cyclization Reaction
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United States of America Patent
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Jan 23, 2025
app pub date -
Jul 8, 2024
filing date -
Jul 8, 2024
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Abstract
Compositions, systems and methods for performing Mannich cyclization reaction and applications thereof are described. Various enzymes, which are referred to as Mannich cyclases, are utilized to catalyze Mannich cyclization with various substrates to yield an azacyclic α-amino acid. Organisms can express or produce a Mannich cyclase via heterologous expression. Methods can be performed to produce a larger molecule, including a therapeutic, using a Mannich cyclase to produce a precursor compound for the larger molecule.
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