SEPARATION METHOD OF ANTIBODY
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United States of America Patent
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Issued Date -
Jan 23, 2025
app pub date -
Nov 14, 2022
filing date -
Nov 14, 2022
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Abstract
An antibody separation method including a step of applying a solution containing an antibody to a column filled with an antibody adsorption material containing an insoluble porous carrier and an Fc-binding protein immobilized on the carrier and adsorbing the antibody onto the antibody adsorption material; and a step of applying a buffer solution to the column and eluting the antibody adsorbed onto the antibody adsorption material with a pH gradient, wherein the pH gradient is a step gradient in which a pH of the buffer solution is lowered in steps, and wherein the magnitude of pH decrease is 0.1 or more and less than 1.
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