Importance

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Abstract

The present disclosure provides methods, assays, kits, and uses thereof for screening candidate therapeutics for their tendency to induce phagocytosis in a cell. In one aspect, the present disclosure provides a method of assaying a candidate compound for its tendency to induce phagocytosis in a cell, the method comprising: contacting the cells with the candidate compound; contacting the cells with a target ligand bound to a fluorescent phagocytosis vesicle reporter having a fluorophore emission wavelength; nuclear staining the cells with a DNA-specific fluorescent stain; and measuring the fluorescence amplitude of the fluorescent phagocytosis vesicle reporter. In another aspect, the present disclosure provides a cell-health assay for testing compounds for their potential for treating neurodegenerative disorders.

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First Claim