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Abstract

A culture method for high-concentration Haematococcus pluvialis includes the following steps: 1) inoculating a Haematococcus pluvialis algae into a first culture medium for culturing for 7-10 days, collecting algal cells, inoculating the algal cells into a new first culture medium for culturing until a dry weight concentration of the algal cells in a Haematococcus pluvialis liquid obtained by culturing is 2.0 g/L or more, and collecting the algal cells, namely motile vegetative cells; 2) inoculating the motile vegetative cells into a second culture medium for culturing for 13-15 days to obtain nonmotile vegetative cells with aged cell walls; and 3) inoculating the nonmotile vegetative cells into a third culture medium for culturing for 10-14 days, inducing the generation of astaxanthin until a content of the astaxanthin is 6% or more, and finishing the culture.

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