METHOD FOR SELECTING TISSUE-SPECIFIC EXTRACELLULAR VESICLE MARKER, TISSUE-SPECIFIC MARKER, AND METHOD FOR PURIFYING SAME
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United States of America
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Jan 9, 2025
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Oct 20, 2022
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Oct 20, 2022
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Abstract
By isolating high purity plasma and serum extracellular vesicles by affinity capture and comparing them with tissue-specific RNA expression data, proteins that were detected in extracellular vesicles and were found to have tissue-specific expression were selected as tissue-specific extracellular vesicle markers. This has allowed the provision of markers with higher specificity, relative to the tissue-specific markers conventionally reported. Use of the tissue-specific markers allows use thereof in diagnosis with high accuracy. Moreover, use of a marker expressed on the extracellular vesicles allows purification of tissue-specific extracellular vesicles with high purity, and they are therefore also useful for drug discovery research and development.
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