MANUFACTURING METHOD OF ARTIFICIAL SKIN USING CELLS DIFFERENTIATED FROM INDUCED PLURIPOTENT STEM CELLS
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United States of America
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Issued Date -
Jan 9, 2025
app pub date -
Jun 29, 2023
filing date -
Jun 29, 2023
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Abstract
The present invention relates to artificial skin using fibroblasts and keratinocytes, and more particularly, to a manufacturing method of artificial skin using cells differentiated from induced pluripotent stem cells (iPSCs). To this end, there is provided a manufacturing method of artificial skin using cells differentiated from iPSCs including the steps of: preparing induced pluripotent stem cells (iPSCs) of a donor (S100); performing differentiating fibroblasts from the iPSCs (S140) and differentiating keratinocytes from the iPSCs (S120) simultaneously or sequentially; injecting the fibroblasts and the keratinocytes using a 3D printer (S160); and manufacturing artificial skin by co-culturing the injected fibroblasts and keratinocytes (S160).
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