Parallel Microfluidic Device for High Throughput Cell Assays in Microdroplets
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United States of America
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Jan 9, 2025
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Sep 20, 2024
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Sep 20, 2024
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Systems and methods for high throughput microdroplet-based single cell assays in microdroplets are provided. The system and methods use parallel devices and switches to enable simultaneous analysis of different cells or cell combinations, or different assay conditions, on a single microfluidic chip. Interconnections between the inlets of individual devices on a common chip enable simultaneous screening of the effect of different combinations of drugs on single cells. The use of an oil inlet and microchannels with matched total flow resistance allows the synchronous generation of droplets with the same dimensions and/or volumes.
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