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Abstract

A sample image generation device includes a memory and a processor. A first image is an image obtained by capturing an image of a sample. A predetermined direction in the first image is a direction in which a virtual observation optical system is present among optical axis directions of the virtual observation optical system. The processor acquires the first image from the memory, divides the first image into a plurality of areas, acquires a refractive index distribution of the sample from the memory, calculates a point spread function using the refractive index distribution, generates second images using the point spread function, combines the second images, and generates a third image corresponding to the first image. In the calculation process, the point spread function of a first area is calculated using the refractive index distribution of each area included in an area group. The first area is an area for which the point spread function is to be calculated. The area group is constituted of a plurality of areas inside a range in which light rays originating from the first area radiate in the predetermined direction.

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First Claim