Robotic Sperm Preparation in an Intelligent Automated In Vitro Fertilization and Intracytoplasmic Sperm Injection Platform
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United States of America
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Dec 26, 2024
app pub date -
Feb 2, 2024
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Feb 2, 2024
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Abstract
A method of artificial-intelligence-based robotic pipetting for spermatozoa preparation includes positioning a vessel containing a semen sample on a staging mechanism, wherein the staging mechanism includes at least one motor to position the vessel at a specified orientation, using an artificial intelligence/machine learning (AI/ML system) system. The method includes confirming a position of the vessel, using the AI/ML system. The method includes directing a robotic pipettor to place a pipette tip within the semen sample at a position specified by the AI/ML system. The method includes aspirating and mixing the semen sample using the robotic pipettor. The method includes receiving an image object from an imaging system that includes a microscopy system, a camera system, and a lighting system. The method includes determining a semen liquefaction state of the semen sample based at least in part on the image object, using the AI/ML system.
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