PRIMER SET, KIT AND METHOD FOR QUALITATIVE AND QUANTITATIVE DETECTION OF SCENTED RICE
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United States of America
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Dec 19, 2024
app pub date -
Oct 12, 2023
filing date -
Oct 12, 2023
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Abstract
A qualitative and quantitative detection primer set, a kit and a detection method for scented rice are provided. The scented rice and non-scented rice are qualitatively analyzed by fluorescence quantitative polymerase chain reaction (PCR), and the scented rice with different adulteration ratios is calculated by a 2-ΔΔCT method, so that the scented rice and the non-scented rice of different varieties can be accurately identified. Primers are designed for specific fragments of the scented rice and the non-scented rice respectively based on the principle of amplification-refractory mutation system (ARMS)-PCR, and qualitative identification of the scented rice can be achieved by agarose gel electrophoresis or fluorescence quantitative PCR according to different laboratory conditions. Combined with internal reference primers for the calculation of gene level by fluorescence quantitative PCR method of scented rice and/or non-scented rice can achieve the adulteration detection of the scented rice quantitatively.
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