PRODUCTION OF ALPHA-1,4-FUCOSYLATED COMPOUNDS
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United States of America
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Dec 19, 2024
app pub date -
Dec 14, 2022
filing date -
Dec 14, 2022
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Abstract
The present invention is in the technical field of synthetic biology, metabolic engineering and cell cultivation. The present invention describes methods for the production of a fucosylated compound comprising Gal-β1,3-[Fuc-α1,4]-GlcNAc using a fucosyltransferase as well as the purification of said fucosylated compound, said fucosyltransferase having alpha-1,4-fucosyltransferase activity on the N-acetylglucosamine (GlcNAc) residue of Gal-β1,3-GlcNAc (lacto-N-biose, LNB) and/or Gal-β1,3-GlcNAc of a saccharide substrate comprising Gal-β1,3-GlcNAc. The present invention also provides a cell for production of a fucosylated compound comprising Gal-β1,3-[Fuc-α1,4]-GlcNAc.
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