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Abstract

A method for preparing beta-carotene from pepper pigment includes: (1) enzymatic hydrolysis of paprika oleoresin to obtain a paprika red pigment hydrolysate; (2) extraction of the paprika red pigment hydrolysate to obtain a paprika red pigment solution; (3) refined filtration to obtain a paprika red pigment filtrate; (4) adsorption: carrying out chromatographic separation with a medium-pressure silica gel chromatographic column, and collecting an effluent from the chromatographic column; (5) elution of beta-carotene; (6) elution of yellow pigments; (7) elution of red pigments; and (8) elution of orange pigments. The invention combines enzymatic hydrolysis with medium-pressure column chromatography for the first time, where chromatographic silica gel with smaller particle size is used for adsorption, and four-stage elution treatment is carried out, so that beta-carotene with a content of more than 20% can be directly separated, and at the same time, reddish paprika red pigment with higher quality is prepared.

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First Claim