METHODS FOR DEVELOPING A CELL LINE FOR PRODUCING VIRUS IN SUSPENSION CELL CULTURE
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United States of America
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Dec 12, 2024
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Oct 27, 2022
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Oct 27, 2022
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Abstract
A method for generating a cell line for producing virus in suspension cell culture. The method comprises generating a plurality of cell clones growing in adherent cell culture, identifying a group of cell clones with high transfection efficiency, generating a plurality of cell clones growing in suspension cell culture and identifying a cell clone suitable for producing virus in suspension cell culture. The method disclosed herein the method can shorten the domestication time from at least 1 month to 7-14 days, and the amount of medium used during the domestication process can also be greatly reduced, reducing production costs.
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