AN IMPROVED PROCESS FOR PURIFICATION OF FUSION PROTEIN
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United States of America
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Dec 5, 2024
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Sep 28, 2022
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Sep 28, 2022
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Abstract
The present invention relates to purification process of pharmacologically active cytotoxic T lymphocyte-associated 4-immunoglobulin (CTLA4-lg) fusion protein by using three column chromatography steps that provides purified form of the fusion protein, substantially free of misfolded species, charge variants and aggregates. A method for purifying a fusion protein from a protein mixture comprising a fusion protein and high molecular weight (HMW) impurity.
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